关键词: 右旋柠檬烯, MCF-7 细胞, 肠道微生物, 抗肿瘤

Abstract: Objective To observe the effect of d-limonene on intestinal microbes in nude mice bearing MCF-7 breast cancer. Method After 30 nude mice were inoculated with MCF-7, dextro-limonene was given at 80 mg / kg, 160 mg / kg, and 320 mg / kg for 6 weeks. Fecal genomic DNA was extracted, and PCR amplification was performed to construct after the library was quantified by Qubit and Q-PCR, the NovaSeq6000 was used for online sequencing, and each sample data was separated from the offline data. After strict filtering, high-quality Clean Tags were obtained, and the chimera sequence was removed to obtain the final after the effective data ( effective tags) , analyze the microbial diversity. Result The bacterial species and abundance decreased after tumor inoculation, and the intestinal flora species and abundance increased after d-limonene treatment. The tumor group was close to the phylum Firmicutes and Bacteroides at the phylum level, and the bisphenol group A and the right number of Bacteroides in the limonene group increased. At the genus level, compared with the tumor control group ( group H ) , Lactobacillus in the bisphenol A and dextro-limonene group decreased significantly, and the isovalerobacter genus increased significantly. Parabacteroides diundi was in the tumor group and bisphenol group A dropped significantly, but the bacteria increased after feeding d-limonene. Conclusion D-limonene significantly affects the number and abundance of intestinal flora in nude mice with breast cancer. Bacteroides and Isovaleri are the dominant flora.

Key words: d-limonene, MCF-7 cells, intestinal microorganisms, anti-tumor